Journal article
A Phenotypic High-Content Screening Assay to Identify Regulators of Membrane Protein Localization
LK Smith, DW Thomas, KJ Simpson, PO Humbert
Assay and Drug Development Technologies | MARY ANN LIEBERT, INC | Published : 2016
DOI: 10.1089/adt.2016.733
Abstract
Correct subcellular localization of proteins is a requirement for appropriate function. This is especially true in epithelial cells, which rely on the precise localization of a diverse array of epithelial polarity and cellular adhesion proteins. Loss of cell polarity and adhesion is a hallmark of cancer, and mislocalization of core polarity proteins, such as Scribble, is observed in a range of human epithelial tumors and is prognostic of poor survival. Despite this, little is known about how Scribble membrane localization is regulated. Here, we describe the development and application of a phenotypic high-content screening assay that is designed to specifically quantify membrane levels of Sc..
View full abstractGrants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This study was supported by a project grant from the NHMRC of Australia (1103871). L.K.S. was supported by an Australian Postgraduate Award. P.O.H. was supported by a Senior Research Fellowship from the NHMRC of Australia (1079133). The Victorian Centre for Functional Genomics (K.J.S.) was funded by the Australian Cancer Research Foundation (ACRF), the Victorian Department of Industry, Innovation and Regional Development (DIIRD), and the Australian Phenomics Network (APN) and it was supported by funding from the Australian Government's Education Investment Fund through the Super Science Initiative and National Collaborative Research Infrastructure Scheme, the Australasian Genomics Technologies Association (AGTA), the Brockhoff Foundation, and the Peter MacCallum Cancer Centre Foundation.